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Not that I believe in the story of Adam and Eve well not all of it that is, we are forgetting the bigger picture here.  God told them not to eat the fruit from that tree, it was considered the forbidden fruit.  Some say it was the tree of knowledge and maybe it was.  Perhaps in the Garden of Eden God was content to let Adam and Eve be dependent on him and therefore wanted them to remain innocent without knowledge.  When they ate from the tree, they were no longer innocent, and now had to be cast out to take care of themselves because they had knowledge to do so.  In a way when I think of this story it sort of like a perent with their own kid.  Parent's raise them try to keep them innocent, but as they grow older and gain knowledge it comes times for them to go out on their own to find their own way.

As for seeking knowledge being a sin because of this story, I don't think so.  I think God wants us to seek knowledge, we now have free will to do so, and doing so does make us more capable of tackling life on a whole.  That is if we use the knowledge for good.